[Purpose/significance] The lack of entrepreneurial ability of college students is an important factor restricting their entrepreneurship. From the Perspective of knowledge evolution, this paper analyzes the reasons for the lack of creativity in entrepreneurial activities and puts forward suggestions, which is of great significance to the construction of entrepreneurial education system for college students. [Method/process] Based on the theory of knowledge evolution, this paper interpreted the evolutionary characteristics, evolutionary patterns and the stimulation of the external environment of college students’ entrepreneurial knowledge. And then, from four aspects of individual, external environment, subject system and education mode, this paper analyzed the factors that restrict the growth of college students’ entrepreneurial ability from the perspective of knowledge evolution. [Result/conclusion] From the view of cultivating college students’ entrepreneurial knowledge, this paper puts forward four reform measures: strengthening the learning environment of college students’ entrepreneurial knowledge, providing interdisciplinary knowledge supply, introducing social practice resources, and pushing forward the reform of education system. It also puts forward some countermeasures and suggestions for colleges and universities to promote entrepreneurial education.