[Purpose/significance] It is the key for the research of enterprise knowledge management to improve the efficiency of enterprise knowledge management by using the advantages of Web 2.0, such as its fastness, public participation and strong interaction. [Method/process] Based on the analysis of the characteristics and main technologies of Web 2.0, this paper discussed the role and application of Web 2.0 related technologies in the enterprise knowledge management, and then put forward the enterprise knowledge management methods based on Web 2.0, including knowledge acquisition method, knowledge classification and organization method, knowledge sharing and evaluation method by usingWeb2 .0. [Result/conclusion] Through the introduction of Web 2.0 related technologies into the knowledge management of enterprises, the research provides convenient and low-cost tools and methods for knowledge management related activities, and helps all users to participate in enterprise knowledge management activities quickly and easily.