[目的/意义] 知识分享已是时代主流，如何管理员工知识分享越来越受到学者和企业管理者重视。员工知识分享是一种社会互动方式，知识分享行为不仅受到动机影响，也会受到所处工作环境影响。[方法/过程] 在理论分析的基础上，提出研究模型和研究假设，设计调查问卷，基于224份调查问卷结果，研究探讨组织气氛对员工知识分享动机的影响。[结果/结论] 研究结果表明：组织气氛对员工知识分享动机具有显著正向影响。组织气氛的友好关系氛围、创新氛围、公平氛围分别对员工知识分享动机的组织奖励动机、互惠性动机、乐趣动机、自我效能感都具有显著正向影响。通过对组织气氛与知识分享动机不同维度作用的探讨，丰富了员工知识分享的相关研究，为中国企业重视组织内部的知识分享环境，提高员工知识分享意愿提供启示。

[Purpose/significance] Knowledge sharing has become the mainstream of the times, and how to manage knowledge sharing has been paid more and more attention by scholars and enterprise managers. Employee knowledge sharing is a kind of social interaction, and the knowledge sharing behavior is not only affected by motivation, but also affected by the working environment. [Method/process] On the basis of theoretical analysis, the research model and research hypothesis were proposed, and the questionnaire was designed. Based on the results of 224 questionnaires, this paper explored the influence of organizational atmosphere on employees' knowledge sharing motivation. [Result/conclusion] The results show that organizational atmosphere has a significant positive effect on employees' knowledge sharing motivation. The friendly relationship atmosphere, innovation atmosphere and fair atmosphere in organizational atmosphere have significant positive influence on the incentive motivation, reciprocity motivation, fun motivation and self-efficacy of employees' knowledge sharing motivation. By discussing the different dimensions of organizational atmosphere and knowledge sharing motivation, this paper enriches the research on employees’ knowledge sharing, and provides enlightenment for Chinese enterprises to attach importance to internal knowledge sharing environment and improve employee knowledge sharing intention.